Self-formed adaptor PCR: a simple and efficient method for chromosome walking.
نویسندگان
چکیده
We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 73 15 شماره
صفحات -
تاریخ انتشار 2007