Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments.

نویسندگان

  • P M Ahrweiler
  • C Frieden
چکیده

A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompanied the fol deletion and is required for survival of a dihydrofolate reductase-deficient strain.

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Construction of a dihydrofolate reductase-deficient mutant of Escherichia coli by gene replacement.

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عنوان ژورنال:
  • Journal of bacteriology

دوره 170 7  شماره 

صفحات  -

تاریخ انتشار 1988