Lensless imaging for point-of-care testing Citation
نویسندگان
چکیده
We show a platform that merges a microfluidic chip with lensless imaging for CD4 T-lymphocyte counting at resource-limited settings. To capture CD4 T lymphocytes, anti-CD4 antibody was immobilized on a microfluidic chip. The captured cells were detected by a charge coupled device (CCD) sensor using lensless shadow imaging techniques. Gray scale shadow images of captured cells on the chip (24 mm × 4 mm × 50 μm) were enumerated in three seconds using an automatic cell counting software. The device achieved 70.2 ± 6.5% capture efficiency, 88.8 ± 5.4% capture specificity for CD4 T-lymphocytes, 96 ± 1.6% CCD efficiency, and 83.5 ± 2.4% overall platform performance (n = 9 devices). This integrated platform has potential for point-of-care testing (POCT) to rapidly capture, image and count specific cell types from unprocessed whole blood.
منابع مشابه
Miniaturized lensless imaging systems for cell and microorganism visualization in point-of-care testing.
Low-cost, robust, and user-friendly diagnostic capabilities at the point-of-care (POC) are critical for treating infectious diseases and preventing their spread in developing countries. Recent advances in micro- and nanoscale technologies have enabled the merger of optical and fluidic technologies (optofluidics) paving the way for cost-effective lensless imaging and diagnosis for POC testing in...
متن کاملLensless digital holographic microscopy and its applications in biomedicine and environmental monitoring.
Optical compound microscope has been a major tool in biomedical imaging for centuries. Its performance relies on relatively complicated, bulky and expensive lenses and alignment mechanics. In contrast, the lensless microscope digitally reconstructs microscopic images of specimens without using any lenses, as a result of which it can be made much smaller, lighter and lower-cost. Furthermore, the...
متن کاملMachine Learning Based Single-Frame Super-Resolution Processing for Lensless Blood Cell Counting
A lensless blood cell counting system integrating microfluidic channel and a complementary metal oxide semiconductor (CMOS) image sensor is a promising technique to miniaturize the conventional optical lens based imaging system for point-of-care testing (POCT). However, such a system has limited resolution, making it imperative to improve resolution from the system-level using super-resolution ...
متن کاملCan We Apply the Control Material from Standard Clinical Chemistry Analyzer for Usage in the Point of Care Testing Analyzer?
متن کامل
Lensless on-chip imaging of cells provides a new tool for high-throughput cell-biology and medical diagnostics.
Conventional optical microscopes image cells by use of objective lenses that work together with other lenses and optical components. While quite effective, this classical approach has certain limitations for miniaturization of the imaging platform to make it compatible with the advanced state of the art in microfluidics. In this report, we introduce experimental details of a lensless on-chip im...
متن کامل