Method for the removal of selected cells from cytological smear preparations for transmission electron microscopy.
نویسندگان
چکیده
The method described depends on fixing additional cytology smear preparations with glutaraldehyde to be used, if needed. Removal of cells from the slide makes use of partially polymerised resins in the usual way but a paper marker is used to localise the area in the resin block which contains the cells to be examined. Slides are placed in a freezing cabinet to facilitate easy separation of the resin block.
منابع مشابه
Human papovavirus in Papanicolaou smears of urinary sediment detected by transmission electron microscopy.
A technique is described whereby inclusion-bearing cells identified by light microscopy in stained smears of urinary sediment were reprocessed for examination in the electron microscope. The nuclei of the abnormal cells were found to contain numerous virus particles, 35 nm in diameter, which morphologically resembled papovaviruses. The technique was applied in this case to identify further the ...
متن کاملDual function of EDTA with silver nanoparticles for root canal treatment–A novel modification
The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococc...
متن کاملComparing Invasive and Non-Invasive of Isolated Shigella flexneri by Electron Microscopy of Cell Culture, SDS-PAGE and Congo Red Method
The aim of this study was to compare invasive and non-invasive strains of Shigella flexneri isolated from Tehran by a 120 kDa protein band by SDS-PAGE, electron microscopy of cell culture and Congo red dye methods. Methods: S. flexneri strains were isolated by standard bacterial methods from fecal specimens of children attending to the 3 children’s hospitals. Phenotype analysis for screening v...
متن کاملLight and electron microscope of grass carp (Ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon
Histopathological effects of different sublethal concentrations (1, 2 and 4 mg/l) of diazinon, an organophosphorus pesticide, on gill, liver, spleen and kidney of grass carp (Ctenopharyngodon idella) were studied after 1, 7, 15, 30 and 45 days post-exposing fishes weighting 850 ± 155 g to the toxicant for 12 hours at 18-22ºC. In addition, tissues of nostril were examined for transmission electr...
متن کاملCanine Periodontal Stem Cells: Isolation, Differentiation Potential and Electronic Microscopic Characterization
Objective- Investigating of the isolation, culture, differentiation potential and electronic microscopic characterization of canine periodontal ligament stem cells (PDLSCs). Design- Experimental in vitro study Animals- Four intact, male, mongrel dogs, 8-10 months-old were selected to collect PDLSCs from their teeth. Procedures- The dogs were anesthetized and the first maxillary and mandibula...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical pathology
دوره 34 12 شماره
صفحات -
تاریخ انتشار 1981