Modified deoxyoligonucleotides stable to exonuclease degradation in serum.
نویسندگان
چکیده
Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoligonucleotides are protected from exonuclease degradation in sera and cell media by simple terminal modifications that maintain high binding activity for the complementary DNA sequence.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 19 4 شماره
صفحات -
تاریخ انتشار 1991