Comparative Genomic Studies of hypothetical proteins in Cyanophages
نویسندگان
چکیده
The Cyanophages are the phages infecting cyanobacteria. They are potential agents for the horizontal gene transfer. The complete genome of 10 known Cyanophages deciphered the presence of various gene sequences for hypothetical proteins whose functions are not yet understood. Our attempt is to predict the structure and function of these hypothetical proteins by the application of computational methods and Bioinformatics. The probable function prediction for the hypothetical proteins was done by using Bioinformatics web tools like CDD-BLAST, INTERPROSCAN, PFAM and COGs by searching protein databases for the presence of conserved domains. While tertiary structures were constructed using PS2 ServerProtein Structure Prediction server. This study revealed presences of functional domain in 258 uncharacterized proteins. These deciphered enzymatic data for hypothetical proteins can be used for the understanding of functional, structural and evolutionary development of cyanophages and its life cycle along with their role in host evolution.
منابع مشابه
Proteome analysis of Cryptosporidium parvum and C. hominis using two-dimentional electrophoresis, image analysis and tandem mass spectrometry
Until recently, Cryptosporidium was thought to be a single species genus. Molecular studies now showthat there are at least 10 valid species of this parasite. Among them, two morphologically identical species, C.hominis and C. parvum are the most pathogenic identified to date and share 97% of identical genomes.Post-genomic analyses is therefore necessary to explore further the...
متن کاملDeciphering the functional role of hypothetical proteins from Chloroflexus aurantiacs J-10-f1 using bioinformatics approach
Chloroflexus aurantiacus J-10-f1 is an anoxygenic, photosynthetic, facultative autotrophic gram negative bacterium found from hot spring at a temperature range of 50-60°C. It can sustain itself in dark only if oxygen is available thereby exhibiting a dark orange color, however display a dark green color when grown in sunlight. Genome of the organism contains total of 3853 proteins out ...
متن کاملConserved ‘Hypothetical’ Proteins: New Hints and New Puzzles
Conserved hypothetical proteins, i.e. conserved proteins whose functions are still unknown, pose a challenge not just to functional genomics but also to general biology. For many conserved proteins, computational analysis provides only a general prediction of biochemical function; their exact biological functions have to be established through direct experimentation. In the few cases when this ...
متن کاملFunctional Annotation of Two Hypothetical Proteins Reveals Valuable Proteins Involved in Response to Salinity: An in silico Approach
Through the exponential development in the specification of sequences and structures of proteins by genome sequencing and structural genomics approaches, there is a growing demand for valid bioinformatics methods to define these proteins function. In this study, our objective is to identify the function of unknown proteins from UCB-1 pistachio rootstock and specify their class...
متن کاملViruses Infecting a Freshwater Filamentous Cyanobacterium (Nostoc sp.) Encode a Functional CRISPR Array and a Proteobacterial DNA Polymerase B
UNLABELLED Here we present the first genomic characterization of viruses infecting Nostoc, a genus of ecologically important cyanobacteria that are widespread in freshwater. Cyanophages A-1 and N-1 were isolated in the 1970s and infect Nostoc sp. strain PCC 7210 but remained genomically uncharacterized. Their 68,304- and 64,960-bp genomes are strikingly different from those of other sequenced c...
متن کامل