Journal of International Academic Research for Multidisciplinary
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چکیده
To test the efficacy of Withaniasomnifera(W.somnifera)on serum lipid profile and protein fractions of heat stressed Japanese quails, a study was conducted using root ethanolic extract and crude powder in two different levels for each. Three hundred 6 weeks old Japanese quail hens was assigned to one of the following five treatments: control T0 (without any addition), T1= daily oral administration with 50mg/kg b.wt. ethanolic extract, T2=daily oral administration with 100mg/kgb.wt. ethanolic extract, T3= daily fed with 1g/kg diet crude powder, T4= daily fed with 2g/kg diet crude powder. At 10 weeks of age high levels of ethanolic extract or crude powder (T1 and T4) decreased significantly serum LDL in comparison with control while non-HDL was decreased significantly in ethanolic extract group as compared to crude powder groups. Treated quails with W.somnifera didn’t affect serum HDL level. At 15 weeks of age, ethanolic extract reduced significantly: triglycerides, VLDL, and serum HDL as compared to control group while non-HDL didn’t affected significantly between treated and control groups. Results of blood serum protein fraction determined by discgel electrophoresis showed significant increasing in albumin and alpha 1globulin percentage in groups supplemented with W.somnifera especially as crude powder. Beta and gamma globulin bands didn’t appear in groups supplemented with root crude powder. It can be concluded from the results of recent study that supplementing heat stressed Japanese quail hens with W.somnifera roots significantly decreased serum LDL at the early stage of egg production whereas at the consequent stage, triglycerides and VLDL was significantly decreased and the percentage of serum albumin, alpha 1 and alpha 2-globulin was significantly increased.
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