Computer Simulation of Capillary Electrophoresis - Analysis of Organic Acids

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a) Run the program HPCESIM. Copy all basic parameters (Equipment, Capillary, Buffer, Sample, Inject and Detect) for electrophoretic separation of mixture of 4 organic acids into table 1. Table 1 Voltage U (kV) Current I (μA) Thermostating temperature T (oC) Capillary length to detector L (mm) ID (inner diameter of capillary) (μm) Separation mode (anionic/cationic) Composition of basic electrolyte Carrier ion: counter ion: pH of basic electrolyte I (ionic strength of basic electrolyte) (mmol/l) Analysed substances Injection volume V (nl) Type of detector Slit width (μm)

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تاریخ انتشار 2014