Some effects of 2-acetylaminofluorene on the dog.

5). The hepatoma is one of the most common neo plasms produced by this drug in the rat. The pur poses of the following experiments were to deter mine the carcinogenic activity of 2-acetylarninofluorene in the dog and to discover some of the changes in metabolism produced by the drug, par ticularly those associated with the induction of hepatomas. Changes in liver function and struc ture are revealed in the dog by an alteration in the urinary uric acid allantoin ratio. If the liver cells are sufficiently destroyed or altered in character, the ratio increases reflecting a reduction in the ac tivity of uricase (2, 6). This ratio was used, there fore, in the experiments described herein, to de tect changes in liver function. Depletion in liver riboflavin and liver protein has been associated with the induction of hepatomas by carcinogens in the rat (7-11). A study of the effects of 2-acetyl aminofluorene on the utilization of riboflavin and proteins by dogs was included, therefore, in the following experiments.