Author's response to reviews Title: Evaluation of a single round polymerase chain reaction assay using dried blood spots for diagnosis of HIV-1 infection in infants in an African setting Authors:
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Evaluation of a single round polymerase chain reaction assay using dried blood spots for diagnosis of HIV-1 infection in infants in an African setting
BACKGROUND The aim of this study was to develop an economical 'in-house' single round polymerase chain reaction (PCR) assay using filter paper-dried blood spots (FP-DBS) for early infant HIV-1 diagnosis and to evaluate its performance in an African setting. METHODS An 'in-house' single round PCR assay that targets conserved regions in the HIV-1 polymerase (pol) gene was validated for use with...
متن کاملEvaluation of HIV-1 polymerase chain reaction (PCR) technique using dried blood spots (DBS) for diagnosis of perinatal transmission of HIV
Background Effective health care delivery to the majority of perinatally exposed infants is hampered by lack of access to accurate HIV diagnosis in infancy. Polymerase chain reaction is the most sensitive test to diagnose HIV-1 infection in children born to HIV seropositive mothers. The purpose of this study was to assess the feasibility and accuracy of using dried blood spot (DBS) technology i...
متن کاملDried blood spots improve access to HIV diagnosis and care for infants in low-resource settings.
Effective health care delivery to the majority of perinatally exposed infants worldwide, including those enrolled in prevention of mother-to-child transmission programs, is hampered by lack of access to an HIV diagnosis in infancy. Dried blood spot collection from young infants for centralized HIV polymerase chain reaction (PCR) testing is attainable in low-resource settings, provided PCR metho...
متن کاملA study to implement early diagnosis of HIV infection in infants born to infected mothers.
A protocol for detecting HIV DNA from specimens collected on filter papers and the effect of storage temperatures on determination of HIV DNA from dried blood spots has been developed and optimized. Blood specimens collected from HIV-1 infected and normal persons were spotted onto blood collection cards (Whatman BFC 180). The HIV DNA was extracted by phenol-chloroform-isoamyl alcohol and was de...
متن کاملLow concentrations of HIV-1 DNA at birth delays diagnosis, complicating identification of infants for antiretroviral therapy to potentially prevent the establishment of viral reservoirs.
Among infants exposed to human immunodeficiency virus type 1 (HIV-1), detection of viral infection at birth was increased by 39% (95% confidence interval, 19%-47%) by increasing DNA input from dried blood spots into polymerase chain reaction. Infants with low concentrations of HIV-1 at birth may be the best target population to evaluate whether immediate antiretroviral therapy can prevent long-...
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