Ku autoantigen is a potential major cause of nonspecific bands in electrophoretic mobility shift assays.
نویسنده
چکیده
منابع مشابه
Cross-resistance to ionizing radiation in a murine leukemic cell line resistant to cis-dichlorodiammineplatinum(II): role of Ku autoantigen.
cis-Dichlorodiammineplatinum(II) (CDDP; cisplatin) is commonly used in combination with ionizing radiation (IR) in the treatment of various malignancies. In vitro, many observations suggest that acquisition of CDDP resistance in cell lines confers cross-resistance to IR, but the molecular mechanisms involved have not been well documented yet. We report here the selection and characterization of...
متن کاملProduction and characterization of recombinant human Ku antigen.
Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...
متن کاملThe glucosaminidase domain of Atl – the major Staphylococcus aureus autolysin – has DNA-binding activity
In this communication, we describe evidence demonstrating the capacity of Atl, the major Staphylococcus aureus autolytic enzyme to bind DNA. Electrophoretic mobility shift assays (EMSA) show that both the Atl protein and the endo-β-N-acetylglucosaminidase (GL) domain were able to bind DNA of nonspecific sequence. The implications of this unexpected observation for the physiology of S. aureus re...
متن کاملبررسی تنوع ژنتیکی لاین های سویا با استفاده از روش الکتروفورز پروتئین دانه
This experiment was conducted to study genetic variation for electrophoretic seed protein patterns and their relations with some seed characteristics such as protein and oil percentages, chemical compositions and 100-seed weight among 270 soybean (Glycine max L. Moench) genotypes. Among different electrophoresis procedures examined, 10% and 4.5% concentrations of acrylamide for resolving and st...
متن کاملبررسی تنوع ژنتیکی لاین های سویا با استفاده از روش الکتروفورز پروتئین دانه
This experiment was conducted to study genetic variation for electrophoretic seed protein patterns and their relations with some seed characteristics such as protein and oil percentages, chemical compositions and 100-seed weight among 270 soybean (Glycine max L. Moench) genotypes. Among different electrophoresis procedures examined, 10% and 4.5% concentrations of acrylamide for resolving and ...
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ورودعنوان ژورنال:
- BioTechniques
دوره 22 2 شماره
صفحات -
تاریخ انتشار 1997