Chromatography of Myoglobin on Diethylaminoethyl Cellulose Columns*

Since the initial description (2, 3) of procedures for the use of cellulose ion exchange columns for the chromatography of proteins, a number of such systems have been developed for specific proteins, including the heme proteins. Thus, Gutter et al. (4) have described the use of carboxymethyl cellulose in a study of various hemoglobins, Rumen (5) has utilized carboxymethyl cellulose for the identification of multiple myoglobins from the seal and Bartsch and Kamen (6) have used diethylaminoethyl cellulose for the isolation of heme proteins from Chromatium. Since the completion of the work described herein, Akeson et al. (7) and Akeson and Theorell (8) have described the use of carboxymethyl cellulose columns for the preparation of myoglobins from rat and horse muscle. This report describes procedures for the chromatography of myoglobins on diethylaminoethyl cellulose columns that are suitable for quantitative determination of myoglobin in extracts, myoglobin-hemoglobin differentiation, and isolation of myoglobin from muscle.