Cloning of dynein intermediate chain cDNA of the newt Cynops pyrrhogaster.

نویسندگان

  • T Yamamoto
  • Y Matsuda
  • T Hikino
  • Y Nakayama
  • S Abé
چکیده

To isolate genes whose expression is up-regulated after initiation of meiosis, we employed an mRNA differential display method using RNA extracted from newt testis fragments in the spermatogonial and spermatocyte stages. We report here isolation of a spermatocyte stage-specific cDNA clone encoding a newt homologue of dynein intermediate chain (IC). The newt dynein IC cDNA was found to encode a polypeptide consisting of 694 amino acid residues with 66.8% and 45.8% amino acid sequence similarity to sea urchin dynein IC3 and Chlamydomonas IC69, respectively. The predicted protein contains five WD repeats and a novel repeated motif in the C-terminal region. Northern blot analysis revealed that newt dynein IC mRNA was expressed in the spermatocyte and round spermatid stages, suggesting that dynein IC plays a role in formation of flagella as well as in meiotic events.

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عنوان ژورنال:
  • DNA research : an international journal for rapid publication of reports on genes and genomes

دوره 8 2  شماره 

صفحات  -

تاریخ انتشار 2001