Isolation and characterization of 8-demethoxy steffimycins and generation of 2,8-demethoxy steffimycins in Streptomyces steffisburgensis by the nogalamycin biosynthesis genes.

Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin.

The biosynthetic gene cluster for the aromatic polyketide steffimycin of the anthracycline family has been cloned and characterized from "Streptomyces steffisburgensis" NRRL 3193. Sequence analysis of a 42.8-kbp DNA region revealed the presence of 36 open reading frames (ORFs) (one of them incomplete), 24 of which, spanning 26.5 kb, are probably involved in steffimycin biosynthesis. They code f...

Isolation and Characterization of Antimicrobial Compound from Marine Streptomyces hygroscopicus BDUS 49

A total of sixty eight actinomycetes were isolated from near sea shore marine environment locations of Bigeum Island, South West coast of South Korea. The majority of these isolates were assigned to the genus Streptomyces of which one isolate showing broad spectrum of antimicrobial was on the basis of their morphological, physiological and biochemical properties. Phylogenetic analysis of the 16...

Isolation and characterization of a phage active against streptomyces griseus

Enhanced topoisomerase II targeting by annamycin and related 4-demethoxy anthracycline analogues.

Targeting topoisomerase II (topo II) is regarded as an important component of the pleiotropic mechanism of action of anthracycline drugs. Here, we show that 4-demethoxy analogues of doxorubicin, including annamycin, exhibit a greater ability to trap topo II cleavage complexes than doxorubicin and some other 4-methoxy analogues. In leukemic CEM cells with wild-type topo II, annamycin induced sub...

Biosynthesis of gold nanoparticles using streptomyces fulvissimus isolate

Objective(s): In recent years, the biosynthesis of gold nanoparticles has been the focus of interest because of their emerging application in a number of areas such as biomedicine. In the present study we report the extracellular biosynthesis of gold nanoparticles (AuNPs) by using a positive bacterium named Streptomyces fulvissimus isolate U from rice fields of Guilan Province, Iran.Materials a...