REVOLUTIONIZE YOUR DESKTOP SEQUENCING WORKFLOW HALOPLEX TARGET ENRICHMENT SYSTEM What a Difference a Day Makes Complete Target Enrichment in Less Than 6 hours

HaloPlex Target Enrichment from FFPE Tissues

The Agilent HaloPlex Target Enrichment System enables fast, simple, and efficient analysis of genomic regions of interest for a large number of samples, covering thousands of exons per sample. It uses single-tube target amplification and removes the need for library preparation to reduce total sample processing time to only 6 hours, without the need for dedicated instrumentation (Figure 1). Hal...

HaloPlex Target Enrichment from FFPE Tissues

The Agilent HaloPlex Target Enrichment System enables fast, simple, and efficient analysis of genomic regions of interest for a large number of samples, covering thousands of exons per sample. It uses single-tube target amplification and removes the need for library preparation to reduce total sample processing time to only 6 hours, without the need for dedicated instrumentation (Figure 1). Hal...

Detection of Rare Variants in Degraded FFPE Samples Using the HaloPlex Target Enrichment System

A combination of targeted enrichment methodologies for whole-exome sequencing reveals novel pathogenic mutations

Whole-exome sequencing (WES) is a useful method to identify disease-causing mutations, however, often no candidate mutations are identified using commonly available targeted probe sets. In a recent analysis, we also could not find candidate mutations for 20.9% (9/43) of our pedigrees with congenital neurological disorder using pre-designed capture probes (SureSelect V4 or V5). One possible caus...

Mung Bean Nuclease Treatment Increases Capture Specificity of Microdroplet-PCR Based Targeted DNA Enrichment

Targeted DNA enrichment coupled with next generation sequencing has been increasingly used for interrogation of select sub-genomic regions at high depth of coverage in a cost effective manner. Specificity measured by on-target efficiency is a key performance metric for target enrichment. Non-specific capture leads to off-target reads, resulting in waste of sequencing throughput on irrelevant re...