Construction of a cDNA microarray derived from the ascidian Ciona intestinalis.

نویسندگان

  • Kaoru Azumi
  • Hiroki Takahashi
  • Yasufumi Miki
  • Manabu Fujie
  • Takeshi Usami
  • Hisayoshi Ishikawa
  • Atsusi Kitayama
  • Yutaka Satou
  • Naoto Ueno
  • Nori Satoh
چکیده

A cDNA microarray was constructed from a basal chordate, the ascidian Ciona intestinalis. The draft genome of Ciona has been read and inferred to contain approximately 16,000 protein-coding genes, and cDNAs for transcripts of 13,464 genes have been characterized and compiled as the "Ciona intestinalis Gene Collection Release I". In the present study, we constructed a cDNA microarray of these 13,464 Ciona genes. A preliminary experiment with Cy3- and Cy5-labeled probes showed extensive differential gene expression between fertilized eggs and larvae. In addition, there was a good correlation between results obtained by the present microarray analysis and those from previous EST analyses. This first microarray of a large collection of Ciona intestinalis cDNA clones should facilitate the analysis of global gene expression and gene networks during the embryogenesis of basal chordates.

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عنوان ژورنال:
  • Zoological science

دوره 20 10  شماره 

صفحات  -

تاریخ انتشار 2003