On-line detection of nonspecific protein adsorption at artificial surfaces.

Detection of non-specific protein adsorption at artificial surfaces by the use of acoustic plate mode sensors

The interaction of proteins with artificial surfaces is important to many medical and biochemical applications. Su h examples involve the incorporation of catheters and prostheses as well as the non-specific adsorption of pharmacological proteins at the walls of a container, which may drastically reduce their activity. A fast analytical tool capable of determining the specific adsorption charac...

Nonspecific protein adsorption at the single molecule level studied by atomic force microscopy.

Liquid tapping atomic force microscopy was used to study the nonspecific adsorption of horse spleen ferritin at a bare gold surface at single molecule resolution. The majority of ferritin molecules adsorbed irreversible on gold surfaces in accordance with the random sequential adsorption (RSA) mechanism frequently used to describe irreversible adsorption processes. However, the time-resolved da...

Composite surface for blocking bacterial adsorption on protein biochips.

The design and fabrication of protein biochips requires characterization of blocking agents that minimize nonspecific binding of proteins or organisms. Nonspecific adsorption of Escherichia coli, Listeria innocua, and Listeria monocytogenes is prevented by bovine serum albumin (BSA) or biotinylated BSA adsorbed on SiO(2) surfaces of a biochip that had been modified with a C(18) coating. Biotiny...

Representation of Adsorption Data for the Case of Energetically Heterogeneous Solid Surfaces Using Artificial Neural Network

QCM-D Study on Relationship of PEG Coated Stainless Steel Surfaces to Protein Resistance

Nonspecific protein adsorption generally occurs on any solid surfaces and usually has adverse consequences. Adsorption of proteins onto a solid surface is believed to be the initial and controlling step in biofouling. Surfaces modified with end-tethered poly(ethylene glycol) (PEG) have been shown to be protein-resistant to some degree. In this study, the adsorption of β-casein and lysozyme was ...