Amino-acid acceptor activity of the "70S-associated" 4S RNA from avian myeloblastosis virus.
نویسندگان
چکیده
The "70S-associated" 4S RNA from avian myeloblastosis virus contains transfer RNA, as determined by aminoacylation. The various amino acids esterified, plus the great number of minor bases found in this "70S-associated" 4S RNA indicate that a sizeable fraction, but not all, of this RNA is transfer RNA.
منابع مشابه
tRNA's associated with the 70S RNA of avian myeloblastosis virus.
The distribtuion of various amino acid tRNA's in the 4S RNA components of avian myeloblastosis virus (AMV) and in 4S RNA prepared from chicken cmbryo cells, chicken myeloblasts, and chicken livers was determined. This was done by aminoacylating the 4S RNA samples with a mixture of 17 radioactive amino acids and subsequently identifying the tRNA-accepted amino acids on an amino acid analyzer aft...
متن کاملMinor base composition of "70S-associated" 4S RNA from avian myeloblastosis virus.
Using a chemical isotope derivative method, we have determined the minor base composition of the "70S-associated" 4S RNA isolated from avian myeloblastosis virus. The minor base composition of this "70S-associated" 4S RNA is strikingly similar to that of the corresponding "free" 4S RNA of the virion. This minor base content, plus the capacity to esterify amino acids, establishes that both of th...
متن کاملExtent of double strandedness in avian myeloblastosis virus RNA.
The extent of double strandedness of avian myeloblastosis virus 70S RNA has been determined from fluorescence measurements of the intercalation of ethidium bromide. We have shown that 50% of the nucleotides of 70S RNA in solution are in a stable helical configuration. This value does not include small helical regions that are too unstable to permit intercalation of the dye. The avian myeloblast...
متن کاملCovalent linkage between ribonucleic Acid primer and deoxyribonucleic Acid product of the avian myeloblastosis virus deoxyribonucleic Acid polymerase.
Initiation of deoxyribonucleic acid (DNA) synthesis by the avian myeloblastosis virus DNA polymerase was previously suggested to involve a ribonucleic acid (RNA) primer, the initial product being a DNA molecule joined by a phosphodiester bond to the RNA primer. The existence and nature of such an RNA-DNA joint was investigated by assaying for transfer of a (32)P atom from an alpha-(32)P-deoxyri...
متن کاملRibonucleic Acid Components of BAI Strain A (Myeloblastosis) Avian Tumor Virus1
Sucrose-density-gradient centrifugation of RNA extracted from HAI strain A virus with phenol revealed 4 distinct com ponents in continuous-flow sjiectrophotometer diagrams. Two of these, not previously described, sedimented at about 16 S and 26 S in association with the components of about 60 S and 5 S reported by others. The 5 S material, like cell transfer RNA, exhibited the amino-acid-accept...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 70 4 شماره
صفحات -
تاریخ انتشار 1973