Whole column fluorescence imaging on a microchip by using a programmed organic light emitting diode array as a spatial-scanning light source and a single photomultiplier tube as detector.
نویسندگان
چکیده
A novel miniaturized, integrated whole-column imaging detection (WCID) system on a microchip is presented. In this system, a program controlled organic light emitting diode (OLED) array was used as a spatial-scanning light source, to achieve imaging by the time sequence of the excited fluorescence. By this mechanism, a photomultiplier tube (PMT) instead of a charge coupled detector (CCD) can be applied to the imaging. Unlike conventional systems, no lenses, fibers or any mechanical components are required either. The novel flat light source provides uniform excitation light without size limitations and outputs a stronger power by pulse driving. The scanning mode greatly reduced the power consumption of the light source, which is valuable for a portable system. Meanwhile, this novel simplified system has a broader linear range, higher sensitivity and higher efficiency in data collection. Isoelectric focusing of R-phycoerythrin (PE) and monitoring of the overall process with WCID were performed on this system. The limit of detection (LOD) was 38 ng mL(-1) or 3.2 pg at 85 nL per column injection of PE. The system provides a technique for WCID capillary isoelectric focusing (cIEF) on chip and can be used for throughput analysis.
منابع مشابه
Fluorometric flow-immunoassay for alkylphenol polyethoxylates on a microchip containing a fluorescence detector comprised of an organic light emitting diode and an organic photodiode.
A compact fluorescence detector was constructed on a microchip from an organic light emitting diode (OLED) as the light source and an organic photodiode (OPD) as the photo-detector and was used in an immunoassay for alkylphenol polyethoxylates (APE). The OLED based on a terbium complex emitted a sharp light at the main wavelength of 546 nm with a full width at half maximum of 9 nm. The incident...
متن کامل290 and 340 nm UV LED arrays for fluorescence detection from single airborne particles.
We demonstrate a compact system, incorporating a 32-element linear array of ultraviolet (290 nm and 340 nm) light-emitting diodes (LEDs) and a multi-anode photomultiplier tube, to the in-flight fluorescence detection of aerosolized particles, here containing the biological molecules tryptophan and NADH. This system illustrates substantial advances in the growth and fabrication of new semiconduc...
متن کاملA collinear light-emitting diode-induced fluorescence detector for capillary electrophoresis.
A novel fluorescence detector based on collinear scheme using a brightness light-emitting diode emitting at 470nm as excitation source is described. The detector is assembled by all-solid-state optical-electronic components and coupled with capillary electrophoresis using on-column detection mode. Fluorescein isothiocyanate (FITC) and FITC-labeled amino acids and small molecule peptide as test ...
متن کاملSynthesis and characterization of Gd2O2 S: Tb3+ phosphor powder for X-ray imaging detectors
Gadolinium oxysulfide phosphor doped with trivalent terbium have been synthesized using urea homogenous precipitation and followed by sulfurization at 800 °C under argon atmosphere. Structural and morphological of synthesized phosphor powder were characterized by x-ray diffraction (XRD), scanning electron microscopy (SEM) and Fourier transform infrared spectrometry (FT-IR). Hexagonal structure ...
متن کاملA portable and autonomous multichannel fluorescence detector for on-line and in situ explosive detection in aqueous phase.
A multichannel fluorescence detector used to detect nitroaromatic explosives in aqueous phase has been developed, which is composed of a five-channel sample-sensor unit, a measurement and control unit, a microcontroller, and a communication unit. The characteristics of the detector as developed are mainly embedded in the sensor unit, and each sensor consists of a fluorescent sensing film, a lig...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Lab on a chip
دوره 7 11 شماره
صفحات -
تاریخ انتشار 2007