Structure in nascent RNA leads to termination of slippage transcription by T7 RNA polymerase.

Structure in Nascent RNA Leads to Termination of Slippage Transcription by T7 RNA Polymerase †Supported by grants 1R01GM55002 from the National Institutes of Health and MCB-9630447 from the National Science Foundation

Studies of RNA release reaction catalyzed by E. coli transcription termination factor rho using isolated ternary transcription complexes.

Protein factor rho catalyzes site-specific termination of transcription in a reaction requiring hydrolysis of nucleoside triphosphate with eventual release of RNA from RNA polymerase and DNA template. We have characterized the rho-catalyzed RNA release reaction using isolated transcription complexes. Transcription complexes containing T7 D111 DNA, RNA polymerase, and 3H-labeled nascent RNA were...

Transcription termination by bacteriophage T7 RNA polymerase at rho-independent terminators.

We have investigated the mechanism of transcription termination by T7 RNA polymerase using templates encoding variants of the transcription-termination structure (attenuator) of the regulatory region of the threonine (thr) operon of Escherichia coli. The thr attenuator comprises the following two distinct structural elements: a G + C-rich inverted repeat, which encodes an RNA hairpin structure,...

Bacteriophage λ N protein inhibits transcription slippage by Escherichia coli RNA polymerase

Transcriptional slippage is a class of error in which ribonucleic acid (RNA) polymerase incorporates nucleotides out of register, with respect to the deoxyribonucleic acid (DNA) template. This phenomenon is involved in gene regulation mechanisms and in the development of diverse diseases. The bacteriophage λ N protein reduces transcriptional slippage within actively growing cells and in vitro. ...

Dissociation of halted T7 RNA polymerase elongation complexes proceeds via a forward-translocation mechanism.

A recent model for the mechanism of intrinsic transcription termination involves dissociation of the RNA from forward-translocated (hypertranslocated) states of the complex [Yarnell WS, Roberts JW (1999) Science, 284:611-615]. The current study demonstrates that halted elongation complexes of T7 RNA polymerase in the absence of termination signals can also dissociate via a forward-translocation...