Evolutionarily Conserved Polyadenosine RNA Binding Protein Nab2 Cooperates with Splicing Machinery To Regulate the Fate of Pre-mRNA
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چکیده
Sharon Soucek, Yi Zeng, Deepti L. Bellur, Megan Bergkessel, Kevin J. Morris, Qiudong Deng, Duc Duong, Nicholas T. Seyfried, Christine Guthrie, Jonathan P. Staley, Milo B. Fasken, Anita H. Corbett Graduate Program in Biochemistry, Cell, and Developmental Biology, Emory University School of Medicine, Atlanta, Georgia, USA; Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA; Graduate Program in Cell and Molecular Biology, The University of Chicago, Chicago, Illinois, USA; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, USA; Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, California, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, California, USA; Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, Georgia, USA
منابع مشابه
The Evolutionarily-conserved Polyadenosine RNA Binding Protein, Nab2, Cooperates with Splicing Machinery to Regulate the Fate of pre-mRNA.
Numerous RNA binding proteins are deposited onto an mRNA transcript to modulate post-transcriptional processing events ensuring proper mRNA maturation. Defining the interplay between RNA binding proteins that couple mRNA biogenesis events is crucial for understanding how gene expression is regulated. To explore how RNA binding proteins control mRNA processing, we investigated a role for the evo...
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Polyadenylation regulation and efficient nuclear export of mature mRNPs both require the polyadenosine-RNA-binding protein, Nab2, which contains seven CCCH Zn fingers. We describe here the solution structure of fingers 5-7, which are necessary and sufficient for high-affinity polyadenosine-RNA binding, and identify key residues involved. These Zn fingers form a single structural unit. Structura...
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تاریخ انتشار 2016