SorCS2 Regulates Dopaminergic Wiring and Is Processed into an Apoptotic Two-Chain Receptor in Peripheral Glia

نویسندگان

  • Simon Glerup
  • Ditte Olsen
  • Christian B. Vaegter
  • Camilla Gustafsen
  • Susanne S. Sjoegaard
  • Guido Hermey
  • Mads Kjolby
  • Simon Molgaard
  • Maj Ulrichsen
  • Simon Boggild
  • Sune Skeldal
  • Anja N. Fjorback
  • Jens R. Nyengaard
  • Jan Jacobsen
  • Dirk Bender
  • Carsten R. Bjarkam
  • Esben S. Sørensen
  • Ernst-Martin Füchtbauer
  • Gregor Eichele
  • Peder Madsen
  • Thomas E. Willnow
  • Claus M. Petersen
  • Anders Nykjaer
چکیده

Balancing trophic and apoptotic cues is critical for development and regeneration of neuronal circuits. Here we identify SorCS2 as a proneurotrophin (proNT) receptor, mediating both trophic and apoptotic signals in conjunction with p75(NTR). CNS neurons, but not glia, express SorCS2 as a single-chain protein that is essential for proBDNF-induced growth cone collapse in developing dopaminergic processes. SorCS2- or p75(NTR)-deficient in mice caused reduced dopamine levels and metabolism and dopaminergic hyperinnervation of the frontal cortex. Accordingly, both knockout models displayed a paradoxical behavioral response to amphetamine reminiscent of ADHD. Contrary, in PNS glia, but not in neurons, proteolytic processing produced a two-chain SorCS2 isoform that mediated proNT-dependent Schwann cell apoptosis. Sciatic nerve injury triggered generation of two-chain SorCS2 in p75(NTR)-positive dying Schwann cells, with apoptosis being profoundly attenuated in Sorcs2(-/-) mice. In conclusion, we have demonstrated that two-chain processing of SorCS2 enables neurons and glia to respond differently to proneurotrophins.

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عنوان ژورنال:
  • Neuron

دوره 82  شماره 

صفحات  -

تاریخ انتشار 2014