Molecular study of Apolipoprotein E gene in familial hypercholesterolemic families
نویسندگان
چکیده
Methodology: Seven hypercholesterolemic families were chosen for this study. Case history was taken and pedigree was created in person by visiting every family. Exon3 and exon4 regions of ApoE cistron were amplified using polymerase chain reaction (PCR).After successful amplification, both citrons were sequenced. Single strand conformation polymorphism (SSCP) results were obtained to support the different pattern of single strand polymorphism of studied samples.
منابع مشابه
Molecular Diagnosis of Familial Hypercholesterolemia
Abstract Background and objectives: Familial hypercholesterolemia (FH) is an autosomal disorder characterized by increased levels of total cholesterol and low density lipoprotein cholesterol. The FH clinical phenotype has been associated with increased risk of coronary heart disease and premature death. The mutation in LDLR gene in most cases is responsible for FH phenotype. Furthermore, other ...
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