Evaluation the Effect of Cimetidine, Estradiol and Vitamin E on Myoglobinuric Renal Toxicity in Rats

Iron from injured muscles can produce acute renal failure. Cytochrome P 450 is one enzyme that involved in iron-related acute renal failure. Inhibition this enzyme may decrease rhabdomyolysisinduced myoglobinuric nephrotoxicity. The aim of this study was comparison effect of cimetidine, estradiol and vitamin E on myoglobinuric renal toxicity in rats. This study was conducted in 6 groups. Normal saline was administrated in group 1(as control). Glycerol 50% was injected in groups 2 -6 and then the vitamin E, estradiol and cimetidine were administrated in these groups. Twenty-four hours after glycerol injection, serum of rats was isolated. Serum concentrations of BUN, creatinine, uric acid, sodium, phosphor and potassium were determined. Kidneys were removed. Renal tissue changes were examined by Hematoxiline and Eosine coloring and with light microscope. Injection of glycerol caused muscular injury and rabdomyolysis and acute renal failure. Because, serum concentration of BUN, cereatinine, sodium, phosphor and uric acid was significantly changed in group 2 compared with group 1. Serum concentration of BUN and cereatinine was significantly decreased in group 3, 5 and 6. Serum concentration of sodium was decreased in group 2 and 4, but increased in group 5. Serum concentration of potassium was significantly increased in group 5 compared to group 2 and 4. The serum level of phosphor and uric acid was significantly decreased in group 3, 5 and 6. The histological changes confirmed biochemical findings. This study shows cimetidine can have protective effect against myoglobinuric renal failure, while estradiol aggravates it.