Small polyribonucleotides with 5'-phosphomonoester end-groups.
نویسندگان
چکیده
Occurrence of D-Glyceric Acid in Tobacco Leaves: J. K. Palmer ... ....... 415 Small Polyribonucleotides with 5'-Phosphomonoester End-Groups: L. A. Heppel, P. J. Ortiz, S. Ochoa ..... ........................... 415 Effect of Cortisone on 17-Ketosteroid Excretion in Patients with Diabetes Mellitus: J. L. Izzo and A. Eilers .... ...................... 417 Effect of Reserpine on Adrenocortical Function in Unanesthetized Dogs: R. H. Egdahl, J. B. Richards, D. M. Hume .... ..................... 418 Note on Murphy's and Rhine's Comments: K. Smith and H. J. Canon.. 418 Fluorescent Thorium Mineral: W. L. Minto ...... ..................... 419
منابع مشابه
The formation of phosphate end groups in the radiolysis of polynucleotides in aqueous solution.
The polynucleotides poly(U), poly(C), poly(A) and poly(G) have been gamma-irradiated in N2O- and N2O/O2 (4:1)-saturated aqueous solutions. Hydroxyl radicals from the radiolysis of water react with the polynucleotides thereby producing among other lesions strand breaks. Strand breakage is connected with the formation of phosphomonoester end groups. Such end groups have been determined by measuri...
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Whitfeld and Markham (1) described a method for the stepwise degradation of polyribonucleotides which involved oxidation of the vicinal hydroxyl groups of the terminal nucleoside residue to a dialdehyde by means of periodate, followed by treatment with glycine at pH 10.5 to remove the terminal base. In this way they converted GpC to 3’-GNP and cytosine. A similar procedure was applied to 5’-AMP...
متن کاملPurification and properties of bacteriophage T4-induced RNA ligase.
An enzyme, purified 300-fold from Escherichia coli infected with bacteriophage T4, catalyzes the conversion of 5'-termini of polyribonucleotides to internal phosphodiester bonds. The reaction requires ATP and Mg(++). For every 5'-(32)P terminus rendered resistant to alkaline phosphatase, an equal amount of AMP and PPi are formed. Various polyribonucleotides are substrates in the reaction; to da...
متن کاملThe Enzymatic Phosphorylation of Nucleic Acids and Its Application to End-Group Analysis
Polynucleotide kinase catalyzes the transfer of a phosphate group from ATP to the 5'-hydroxyl termini of polynucleotides. Selective labeling of the 5'-hydroxyl termini of DNA with polynucleotide kinase has been used to study the number and the identity of the 5'-terminal residues of bacteriophage DNA's, and to examine the nature of the phosphodiester bond cleavages produced by endonucleases and...
متن کاملCovalent attachment of polyribonucleotides to polydeoxyribonucleotides catalyzed by deoxyribonucleic acid ligase.
DNA ligase isolated from Escherichia coli or phage T4-infected E. coli catalyzes the covalent joining of the 5’-phosphate termini of polydeoxyribonucleotides and 3’-hydroxyl termini of polyribonucleotides. This reaction occurs with poly(dA) and poly(A) in the presence of poly(dT). The 5’-phosphate terminus of poly[d(A-T)] can be linked by an intramolecular reaction to its 3’-hydroxyl terminus s...
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ورودعنوان ژورنال:
- Science
دوره 123 3193 شماره
صفحات -
تاریخ انتشار 1956