Cutting edge: urease release by Helicobacter pylori stimulates macrophage inducible nitric oxide synthase.

نویسندگان

  • Alain P Gobert
  • Benjamin D Mersey
  • Yulan Cheng
  • Darren R Blumberg
  • Jamie C Newton
  • Keith T Wilson
چکیده

Inducible NO synthase (iNOS) expression and production of NO are both up-regulated with Helicobacter pylori infection in vivo and in vitro. We determined whether major pathogenicity proteins released by H. pylori activate iNOS by coculturing macrophages with wild-type or mutant strains deficient in VacA, CagA, picB product, or urease (ureA(-)). When filters were used to separate H. pylori from macrophages, there was a selective and significant decrease in stimulated iNOS mRNA, protein, and NO(2)(-) production with the ureA(-) strain compared with wild-type and other mutants. Similarly, macrophage NO(2)(-) generation was increased by H. pylori protein water extracts of all strains except ureA(-). Recombinant urease stimulated significant increases in macrophage iNOS expression and NO(2)(-) production. Taken together, these findings indicate a new role for the essential H. pylori survival factor, urease, implicating it in NO-dependent mucosal damage and carcinogenesis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Interactions between inducible nitric oxide and other inflammatory mediators during Helicobacter pylori infection.

BACKGROUND Recent studies in both humans and animal models strongly suggest the contribution of the host immune response to Helicobacter pylori-related disease. Inducible nitric oxide synthase has been shown to be up-regulated in the gastric epithelium during H. pylori gastritis, suggesting a role in inflammation. MATERIALS AND METHODS C57BL/6 wild-type and inducible nitric oxide synthase gen...

متن کامل

Nitric Oxide Synthase Translation in by Attenuating Inducible Helicobacter pylori Arginase II Restricts Host Defense to

متن کامل

Enhanced M1 Macrophage Polarization in Human Helicobacter pylori-Associated Atrophic Gastritis and in Vaccinated Mice

BACKGROUND Infection with Helicobacter pylori triggers a chronic gastric inflammation that can progress to atrophy and gastric adenocarcinoma. Polarization of macrophages is a characteristic of both cancer and infection, and may promote progression or resolution of disease. However, the role of macrophages and their polarization during H. pylori infection has not been well defined. METHODOLOG...

متن کامل

Role of gamma interferon in Helicobacter pylori induction of inflammatory mediators during murine infection.

Gamma interferon (IFN-gamma) has been proposed to play an important role in Helicobacter-related gastritis. Using the IFN-gamma gene knockout (IFN-gamma(-/-)) mouse model and a murine gastric epithelial cell line, GSM06, we demonstrated that Helicobacter pylori maximally induced macrophage inflammatory protein-2 (MIP-2) and inducible nitric oxide synthase (iNOS) mRNA only in wild-type mice. MIP...

متن کامل

Developed Method Application for Nitrite Ion (NO2¯ ) Analysis of Tib -186 Macrophage Like Cell Lines by Rapid Isocratic HPLC System with High Sensitive Glassy Carbon Electrochemical Detector

A rapid isocratic method of high performance liquid chromatography system (HPLC) with a glassy carbon working electrode of electrochemical detector is set up for quantitative detection of  trace amount of nitrite ion (NO2¯) in aqueous protein containing cell lysate, cell media, plasma, serum, urine and other body fluids. The solid extraction  reversed phase cartridges ...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of immunology

دوره 168 12  شماره 

صفحات  -

تاریخ انتشار 2002