NoteA Host - Vector System fora Cellulose - ProducingAcetobacter S rain
نویسندگان
چکیده
4002bp, contained an AT-rich region and eneoded several open reading frfimes, as deduced by the comp]ete nucleotide sequence. One of the plltative open reading frames showed homology with replication proteins of other plasmids. A shuttle vector of Escherichia coti and this strain was constructed by connecting pAH4 to pUCI8. Electroporation of the shllttle vector into the strain yielded 1 .7 x 10i ampicillin resistant transformants per pg DNA. The shuttle plasmid was yery stably maintained in the strain.
منابع مشابه
Construction of T-vector derived from pBluescript ΙΙ SK with a positive selection marker, a rapid system for cloning
A rapid DNA cloning system is a research interest of many scientists. TA cloning is one of the methods used for the cloning of PCR-amplified DNA molecules. The TA cloning method is a convenient and labor-saving replacement to traditional, restriction enzyme-mediated cloning strategies. A T-vector called pBlueskript ΙΙ SK-1 with the lethal gene ccdB was designed to construct a positive selection...
متن کاملThe social construction of acid rain
There is currently a great deal of discussion in various national and international fora about how to design global change research programmes (for an overview of part of the terrain see Price).’ American policy analysts often invoke the National Acid Precipitation Project (NAPAP) as a textbook example of how not to do policy relevant research (ses for example US Congress, Offlce of Technology ...
متن کاملIn vitro Antimicrobial Effect of Probiotic Films Based on Carboxymethyl Cellulose-Sodium Caseinate Against Common Food-Borne Pathogenic Bacteria
Introduction: Consumption of appropriate amount of probiotic microorganisms via food products have health benefits on the host. In recent years, there has been a significant increase in research on the characterization and verification potential use of probiotic films in food industry. The aim of the current study was to investigate in vitro antimicrobial property of p...
متن کاملBiodiversity Can Help Prevent Malaria Outbreaks in Tropical Forests
BACKGROUND Plasmodium vivax is a widely distributed, neglected parasite that can cause malaria and death in tropical areas. It is associated with an estimated 80-300 million cases of malaria worldwide. Brazilian tropical rain forests encompass host- and vector-rich communities, in which two hypothetical mechanisms could play a role in the dynamics of malaria transmission. The first mechanism is...
متن کاملEffect of Polyanionic Cellulose Polymer Nanoparticles on Rheological Properties of Drilling Mud
Polyanionic cellulose polymer is used as an additive in drilling mud in order to decrease water loss and mudcake- thickness. In this study effect of bulk and nanosize polyanionic cellulose on water loss and mud-cakethickness in mud drilling is investigated. Polyanionic cellulose nanoparticles are made by using of ball milling. Size of nanoparticles is measured by Particle size analyzer. Polyani...
متن کامل