Use of the Spin-Trapping Method for the Study of Stable Radicals Produced in Solid Dihydrothymine
نویسنده
چکیده
ESR spin-trapping method has been used for the study of radiation-induced radicals in· powdered dihydrothymine. Four approximately equal small proton couplings (AH' = 0.35 mT), a larger proton coupling (AH = 0.195 mT) and a nitrogen coupling (AN = = 0.295 mT) are attributed to the nitroxide radical formed by reaction of the spin trap with 6-dihydrothymyl radical upon dissolving of dihydrothymine. In the pyrimidine bases of nucleic acid and a number of related compounds irradiated in solid state and dissolved, no signs of the trapped radicals were observed.
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