Technical aspects of the radioimmunoassay of thyrotrophin.
نویسندگان
چکیده
There are two main animal sources; rabbit antihuman TSH available from the National Pituitary Agency (NPA) or the Medical Research Council (MRC) and guinea-pig antihuman TSH prepared locally. Franchimont (1972) has also reported the use of antibodies to porcine TSH which crossreact with human TSH but this reagent is not generally available. Locally produced guinea-pig anti-TSH is used at a final dilution of 1: 600 000 which produces 50-70%
منابع مشابه
Radioimmunoassay of human serum thyrotrophin.
The double antibody radioimmunoassay of serum thyroid-stimulating hormone (TSH) allows measurement of circulating levels of the hormone in most normal subjects. The serum TSH level in normal subjects is 1.6 +/- 0.8muU/ml. Patients with non-toxic goitre and acromegaly have normal TSH levels. Values are always raised in hypothyroid patients (with primary thyroid disease) and are significantly low...
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CLINICAL CHEMISTRY, Vol. 27, No. 3, 1981 505 B., Radioimmunoassay of serum thyrotrophin: Sensitivity and specificity. J. Gun. Endocrinol. 33,768-774 (1971). 2. Sluiter, W. J., Van Kersen, F., Van Zanten, A K., et al., Clin. Chim. Acta 42, 255-262 (1972). 3. Wehmann, R. E., Rubenstein, H. A., and Nisula, B. C., A sensitive convenient radioimmunoassay procedure which demonstrates that serum hTSH ...
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The response to an intravenous dose of 200 microng of thyrotrophin releasing hormone (TRH) has been studied by estimating, by radioimmunoassay, baseline levels followed by further estimations of thyroid stimulating hormone (TSH) 20 minutes after the injection and triiodothyronine (T3) three hours after the injection in 112 patients referred for routine thyroid assessment. Comparison of diagnost...
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Serum thyrotrophin (TSH) was estimated by double-antibody radioimmunoassay in 200 children aged 2 months to 16 years with normal thyroid function. There was no apparent variation in TSH with age or sex and only 4 children had TSH levels greater than 5 muU/ml. High TSH values were obtained in 9 children with primary hypothyroidism, in 3 children with thyroiditis, and in one girl with a lingual t...
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The value as a thyroid function test of a new, rapid, and highly sensitive immunoradiometric assay for thyroid stimulating hormone (TSH) was assessed in 188 consecutive new patients with suspected hyperthyroidism. The diagnosis was made on clinical grounds and on the basis of serum total triiodothyronine and thyroxine concentrations and the response of TSH to thyrotrophin releasing hormone (TRH...
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 28 3 شماره
صفحات -
تاریخ انتشار 1975