Adhesion of Platelets to Human Artery Subendothelium: Effect of Factor Vill-von Willebrand Factor of Various Multimeric Composition

نویسندگان

  • Jan J. Sixma
  • Kjell S. Sakariassen
  • Nel H. Beeser-Visser
  • Mieke Ottenhof-Rovers
  • Piet A. Bolhuis
چکیده

The relationship between the multimeric size of factor VIII-von Willebrand factor (FVlll-vWF) and the support of platelet adhesion to subendothelium was studied in an annular perfusion chamber, employing human renal and umbilical arteries. Commercial factor VIII concentrates containing multimers of low molecular weight that had been shown not to correct the bleeding time upon infusion into patients with von Willebrand’s disease did not support platelet adhesion in the perfusion chamber. Cryoprecipitate and two experimental FVlll-vWF concentrates contaming muftimers of high molecular weight supported platelet adhesion. Factor Vlll-vWF purified from cryoprecipitate was subdivided into three fractions of different molecular weights (6.0-14.0. 4.0-9.0. and 3.07.5 x i05 dalton). These fractions appeared to bind equally

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Adhesion of platelets to human artery subendothelium: effect of factor VIII-von Willebrand factor of various multimeric composition.

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تاریخ انتشار 2005