D1/D2 domain of large-subunit ribosomal DNA for differentiation of Orpinomyces spp.

نویسندگان

  • Sumit S Dagar
  • Sanjay Kumar
  • Priti Mudgil
  • Rameshwar Singh
  • Anil K Puniya
چکیده

This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 77 18  شماره 

صفحات  -

تاریخ انتشار 2011