Stool Antigen Tests for the Detection of Helicobacter Pylori in Children
نویسندگان
چکیده
Stool specimens from 102 children (median age = 6.0 years; Helicobacter pylori in biopsy specimens, n = 43) were tested for H. pylori with the monoclonal antibody-based enzyme immunoassay HpStar. In 28 cases, the specimen was obtained with a rectal cotton swab during endoscopy. The sensitivity and specificity of the HpStar were 0.95 and 0.90, respectively, as compared with biopsy-based methods. The rectal cotton swab specimen gave accurate results in 24 of the 28 cases. Storage of specimens for 1, 3, or 7 days (n = 6) had a minor effect on the results, but one specimen's originally positive value on borderline turned negative when it was kept in room temperature. Forty-eight specimens were tested in parallel with all 3 tests used: HpStar, Premium Platinum HpSA, and the rapid test ImmunoCardSTAT! The accuracy rates of the tests were 98% for the HpSA and HpStar and 96% for the ImmunoCardSTAT! The performance of the stool antigen tests was excellent in young children.
منابع مشابه
Diagnostic Accuracy of Polyclonal Stool Antigen for Detection of Helicobacter Pylori Infection in Children
Introduction: Helicobacter pylori infection has various clinical features. One of the most common presentations of this infection is upper abdominal pain. Complications such as gastritis, gastric ulcer, gastric carcinoma and MALT lymphoma mandate early diagnosis of H.P infection by a low cost and non invasive manner. The aim of this study was to evaluate the diagnostic role of H.P stool antige...
متن کاملPervalance of Helicobacter pylori stool antigen in children with chronic abdominal pain
Background and Objective: Chronic and recurrent abdominal pain affect about 7-20% of children in school age, and it is responsible for 2-4% of children's medical referrals. Helicobacter pylori infection in children can cause gastrointestinal problems in childhood, and the lack of treatment and eradication can lead to serious complications. This study was done to evaluate the prevalence of Helic...
متن کاملDetection of Helicobacter Pylori Diagnostic Antigens in the Stool of Infected Patients
Background: Some antigens of H. pylori are excreted into the stool of infected people. These antigens can be used to detect the infection by immunoassays such as ELISA. Our aim was to identify these antigens by immunoblotting and affinity chromatography techniques. Methods: Four different antigenic preparations, namely, whole cell sonicate (WCS), outer membrane proteins (OMPs), cytoplasmic anti...
متن کاملEvaluation the helicobacter pylori infection in asthmatic children compared to control group
Background: Asthma is a highly prevalent chronic respiratory disease in children. Moreover, the underlying causes of asthma exacerbation are important because they are effective in controlling and preventing asthma. The aim of this study was to evaluate the helicobacter pylori infection in controlled and uncontrolled asthmatic children compared to healthy children. Methods: This case- contro...
متن کاملDiagnosis of Helicobacter pylori infection by invasive and noninvasive tests
Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen tests with polymerase chain reaction (PCR) for detection of H. pylori, and correlate the diagnostic m...
متن کاملThe Effect of Red Rose Extract on Helicobacter Pylori Eradication: A Randomized Controlled Clinical Trial
Background Helicobacter pylori (H. pylori) is believed to be the most common bacterial infection worldwide. Herein, we aimed to investigate the effect of red rose extract on H. pylori eradication in 9 to 15 year-old children. Materials and Methods In this randomized controlled clinical trial,by the convenience sampling method...
متن کامل