Innovative Methodology Microchip amplifier for in vitro, in vivo, and automated whole cell patch-clamp recording
نویسندگان
چکیده
Reid R. Harrison, Ilya Kolb, Suhasa B. Kodandaramaiah, Alexander A. Chubykin, Aimei Yang, Mark F. Bear, Edward S. Boyden,* and Craig R. Forest* Intan Technologies, Los Angeles, California; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia; Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts; McGovern Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts; Department of Biological Sciences, Purdue University, West Lafayette, Indiana; Howard Hughes Medical Institute, The Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts; and George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia
منابع مشابه
Microchip amplifier for in vitro, in vivo, and automated whole cell patch-clamp recording.
Patch clamping is a gold-standard electrophysiology technique that has the temporal resolution and signal-to-noise ratio capable of reporting single ion channel currents, as well as electrical activity of excitable single cells. Despite its usefulness and decades of development, the amplifiers required for patch clamping are expensive and bulky. This has limited the scalability and throughput o...
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The patch-clamp recording technique, which measures ionic currents under voltage clamp, was designed to study small patches of membranes in which near perfect control of the transmembrane voltage can be achieved readily. The recent application of patch-clamp methodology to the analysis of whole-cell current actually defies many of the original design requirements. Nevertheless, whole-cell recor...
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Whole-cell patch-clamp electrophysiological recording is a powerful technique for studying cellular function. While in vivo patch-clamp recording has recently benefited from automation, it is normally performed "blind," meaning that throughput for sampling some genetically or morphologically defined cell types is unacceptably low. One solution to this problem is to use two-photon microscopy to ...
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