Physiological Studies on Spore Germination, with Special Reference to Clostridium botulinum: IV. Inhibition of Germination by Unsaturated C(18) Fatty Acids.

Physiological studies on spore germination, with special reference to Clostridium botulinum; carbon dioxide and germination, with a note on carbon dioxide and aerobic spores.

Functional Characterisation of Germinant Receptors in Clostridium botulinum and Clostridium sporogenes Presents Novel Insights into Spore Germination Systems

Clostridium botulinum is a dangerous pathogen that forms the highly potent botulinum toxin, which when ingested causes a deadly neuroparalytic disease. The closely related Clostridium sporogenes is occasionally pathogenic, frequently associated with food spoilage and regarded as the non-toxigenic equivalent of Group I C. botulinum. Both species form highly resistant spores that are ubiquitous i...

Diversity of the Germination Apparatus in Clostridium botulinum Groups I, II, III, and IV

Clostridium botulinum is a highly dangerous pathogen that forms very resistant endospores that are ubiquitous in the environment, and which, under favorable conditions germinate to produce vegetative cells that multiply and form the exceptionally potent botulinum neurotoxin. To improve the control of botulinum neurotoxin-forming clostridia, it is important to understand the mechanisms involved ...

Physiological Studies on Spore Germination with Special Reference to Clostridium botulinum: I. Development of a Quantitative Method.

By far the majority of studies hitherto made on germination of bacterial spores have employed the appearance of visible turbidity as the criterion of germination. Obviously this technique can reveal no quantitative characteristics of the germination process and is therefore of value only in establishing that some germination does or does not take place. Even here its value may be questioned, si...

Inhibition of germinant binding by bacterial spores in acidic environments.

Commitment to germinate occurred in both Clostridium botulinum and Bacillus cereus spores during 0.5 min of exposure to 100 mM L-alanine or L-cysteine, measured by the inability of germination inhibitors (D form of amino acid) to inhibit germination. Spore germination at pH 4.5 was inhibited because the germinant did not bind to the trigger sites. C. botulinum spores exposed to 100 mM L-alanine...