Detection of anti-human immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen.

نویسندگان

  • K Hashinaka
  • S Hashida
  • K Hirota
  • A Saitoh
  • A Nakata
  • H Shinagawa
  • S Oka
  • K Shimada
  • E Ishikawa
چکیده

Anti-human immunodeficiency virus type 1 immunoglobulin G in urine was detected by an immunoassay with reverse transcriptase as the antigen and beta-D-galactosidase as the label; this immunoassay was 30-fold more sensitive than the previous immunoassay with peroxidase as the label. The sensitivity and specificity were both 100%. The lowest signal for asymptomatic carriers was 20-fold higher than the highest signal for seronegative subjects.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 32 3  شماره 

صفحات  -

تاریخ انتشار 1994