UV spectrophotometric assay of Famotidine formulations

Famotidine belongs to H2-receptor antagonist. It is used widely for the treatment of treatment of (GERD) gastro-esophageal reflux disease and gastric ulceration duodenal ulcer, stress ulcer. A simple, efficient spectrophotometric method for the assay of famotidine has been developed for estimation of famotidine formulations. Comparision of three different brands of famotidine (nocid, famulcer and optifam) has also been done. The assay is carried out in lambda max at about 260 nm using water as solvent. Different formulations of drug was dissolved in water to prepare solutions containing famotidine 20 mg. Similarly, a sample of ground tablets of different brand were dissolved in water and dilutions were made in the range of 200-12.5 μgmL. The absorbance of sample preparation was measured at 260 nm against the water (blank solvent) and the assay was determined by the absorbance of each brand. Our results reveals that among all the three brands of famotidine (nocid, famulcer and optifam) famulcer shows highest percentage assay 111.44%. Calibration curves were linear over the range of 12.5-200 μg mLwith a correlation coefficient ±0.92. Intra and inter-run precision and accuracy results were 98 to 102%. Proposed method was selective, accurate and precise therefore we can use for routine assay as well as quality control and clinical study.