Purification and Properties of 5-Enolpyruvylshikimate-3-Phosphate Synthase from Dark-Grown Seedlings of Sorghum bicolor.

Site-Directed Mutagenesis, Expression and Biological Activity of E. coli 5-Enolpyruvylshikimate 3-Phosphate Synthase Gene

Site-directed mutagenesis (SDM) as a powerful technique was used to change two important and conserved amino acids in 5-enolpyruvylshikimate 3- phosphate synthase (EPSPS) gene of E. coli. The mutations changed glycine 96 to alanine and alanine 183 to threonine. These two amino acids are very important for intraction of the wide spectrum herbicide, glyphosate, to EPSP synthase enzymes. By design...

Progress in cloning, expression and purification of 5-enolpyruvylshikimate-3-phosphate synthase from pathogens causing meningitis.

Influence of Sample Treatment on Apparent Hydrocyanic Acid Potential of Sorghum Leaf Tissue

When dhurrin [p-hydroxy-(S)-mandelonitrile-tJ-D-glucoside], the cyanogenic glucoside of sorghum [Sorghum bicolor (L.) Moench], is hydrolyzed by autoclaving, p-hydroxybenzaldehyde (P-HB) is released. The spectrophotometric determination of pHB concentration in autoclaved sorghum leaf extracts provides a measure of the hydrocyanic acid potential (HCN-p) of leaf tissue. Extracts of field-grown sor...

Purification and Partial Characterization of Host-Specific Toxins

WOLPERT, T. J., and L. D. DUNKLE. 1980. Purification and partial characterization of host-specific toxins produced by Periconia circinata. Phytopathology 70:872-876. Host-specific toxins isolated from culture filtrates of Periconia circinata properties of polyamines. HPLC resolved each of those toxic fractions into inhibited root growth of the susceptible sorghum genotype by 50% at 1 two compou...

Sequence Requirements of the 5-Enolpyruvylshikimate-3-phosphate Synthase 5[prime]-Upstream Region for Tissue-Specific Expression in Flowers and Seedlings.

We have analyzed expression from deletion derivatives of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) 5[prime]-upstream region in transgenic petunia flowers and seedlings. In seedlings, expression was strongest in root cortex cells and in trichomes. High-level expression in petals and in seedling roots was conferred by large (>500 base-pair) stretches of sequence, but was lost when s...