Effect of Signal Compounds on Andrographolide in the Hairy Root Cultures of Andrographis Paniculata

E-mail: [email protected] ABSTRACT: Andrographolide is one of the main active principles in Andrographis paniculata .It exhibits enormous pharmacological effects. Due to this immense value it required more level in pharmaceutical to satisfy the global need. Because it present in a very low level in normal regenerated plant of Andrographis paniculata. Micro propagation is a proven method for efficient invitropropagation of medicinal plants and for commercial exploitation of valuable plant derived pharmaceuticals. Hence in this present study aimed that to enhance the andrographolide content through hairy root culture techniques and elicitation process. Hairy root cultures induced in Andrographis paniculata used for determining the effect of signal compound on andrographolide production. Methyl jasmonate and salicylic acid were used in varying concentration and introduced into the 10d old hairy root cultures. HPLC studies are revealed that methyl jasmonate at 100 mM concentration shows higher efficacy (5fold/25d) than other increasing concentrations of same. Salicylic acid shows 8 fold (100mM) enhancements than hairy root cultures (control).