Evidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/a-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp
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چکیده
Evidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/a-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp. V. GRACE MATHESON, LARRY J. FORNEY,* YUICHI SUWA,† CINDY H. NAKATSU,‡ ALAN J. SEXSTONE, AND WILLIAM E. HOLBEN§ Department of Plant Pathology and Environmental Microbiology, West Virginia University, Morgantown, West Virginia 26506, and Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824
منابع مشابه
Evidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/(alpha)-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp.
We characterized the gene required to initiate the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) by the soil bacterium Burkholderia sp. strain TFD6, which hybridized to the tfdA gene of the canonical 2,4-D catabolic plasmid pJP4 under low-stringency conditions. Cleavage of the ether bond of 2,4-D by cell extracts of TFD6 proceeded by an (alpha)-ketoglutarate-dependent reaction, characte...
متن کاملCharacterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC.
The findings of previous studies indicate that the genes required for metabolism of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-D) are typically encoded on broad-host-range plasmids. However, characterization of plasmid-cured strains of Burkholderia sp. strain RASC, as well as mutants obtained by transposon mutagenesis, suggested that the 2,4-D catabolic genes were located on the chromoso...
متن کاملCharacterization of a Chromosomally Encoded 2,4-Dichlorophenoxyacetic Acid/a-Ketoglutarate Dioxygenase from Burkholderia sp. Strain RASC
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Partial Purification and Characterization of 2,4- Dichlorophenoxyacetic Acid Degrading Bacteria Harboring Alpha Ketoglutarate Dioxgenase Enzyme
The catabolic adaptability demonstrated by microorganisms is important in the bioremediation of polluted environments. 2,4-dichlorophenoxyacetic acid/α-ketoglutarate dioxygenase is the first enzyme in the catabolic pathway for the 2,4dichlorophenoxyacetic acid (2,4-D). In the present study, dioxygenase enzymes were inducible and the serial adaptation of four studied bacterial isolates steadily ...
متن کاملAlcaligenes eutrophus JMP134 "2,4-dichlorophenoxyacetate monooxygenase" is an alpha-ketoglutarate-dependent dioxygenase.
The Alcaligenes eutrophus JMP134 tfdA gene, encoding the enzyme responsible for the first step in 2,4-dichlorophenoxyacetic acid (2,4-D) biodegradation, was overexpressed in Escherichia coli, and several enzymatic properties of the partially purified gene product were examined. Although the tfdA-encoded enzyme is typically referred to as 2,4-D monooxygenase, we were unable to observe any reduct...
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