Addition of a homologous internal control to a real-time PCR assay for detection of Bordetella pertussis.

نویسندگان

  • Christoph Koidl
  • Michael Bozic
  • Jörg Berg
  • Markus Stöcher
  • Gerhard Mühlbauer
  • Egon Marth
  • Harald H Kessler
چکیده

peroxide generated during cellular insulin stimulation is integral to activation of the distal insulin-signaling cascade in 3T3–L1 adipocytes. J Biol Chem 2001;276:42662–9. 7. Pomytkin IA, Kolesova OE. Effect of insulin on the rate of hydrogen peroxide generation in mitochondria. Bull Exp Biol Med 2003;135: 541–2. 8. Xu L, Badr MZ. Enhanced potential for oxidative stress in hyperinsulinemic rats: Imbalance between hepatic peroxisomal hydrogen peroxide production and decomposition due to hyperinsulinemia. Horm Metab Res 1999;31: 278–82. 9. Robertson RP, Harmon J, Tran PO, Tanaka Y, Takashi H. Glucose toxicity in -cells: type 2 diabetes, good radicals gone bad, and the glutathione connection. Diabetes 2003;52:581–7. 10. Hansen LL, Ikeda Y, Olsen GS, Busch AK, Masthof L. Insulin signaling is inhibited by micromolar concentration of H2O2: evidence for the role of H2O2 in tumor necrosis factor -mediated insulin resistance. J Biol Chem 1999; 274:25078–84. 11. Bloch KO, Vorobeyechi M, Yavrians K, Vardi P. Selection of insulin-producing rat insulinoma (RINm) cells with improved resistance to oxidative stress. Biochem Pharmacol 2003;65:1797–805. 12. Sindhu RK, Koo JR, Roberts CK, Vazioori ND. Dysregulation of hepatic superoxide dismutase, catalase, and glutathione peroxidase in diabetes: responses to insulin and antioxidant therapies. Clin Exp Hypertens 2004; 26:43–53. 13. Weidig P, McMaster D, Bayraktutban U. High glucose mediates pro-oxidant and antioxidant enzyme activities in coronary endothelial cells. Diabetes Obes Metab 2004;6:432–41. 14. Manea A, Constatinescu E, Popov D, Raicu M. Changes in oxidative balance in rat pericytes exposed to diabetic conditions. J Cell Mol Med 2004;8:117–26. 15. Góth L, Eaton JW. Hereditary catalase deficiencies and increased risk of diabetes. Lancet 2000;356:1820–1. 16. Forsberg H, Borg LAH, Cagliero E, Eriksson UJ. Altered levels of scavenging enzymes in embryos subjected to a diabetic environment. Free Radic Res 1996;24:451–9. 17. Wender-Ozegowska E, Kozlik J, Bizcysko R, Egowski S. Changes of oxidative stress parameters in diabetic pregnancy. Free Radic Res 2004;38:798–803. 18. Vitai M, Góth L. Reference ranges of normal blood catalase activity and levels in familial hypocatalasemia in Hungary. Clin Chim Acta 1997;261: 35–42. 19. Góth L. A new type of inherited catalase deficiencies: its characterization and comparison to the Japanese and Swiss type of acatalasemia. Blood Cells Mol Dis 2001;27:512–7. 20. Forsberg L, Lyreruas L, Faire U, Morgenstern R. A common functional C-T substitution polymorphism in the promoter region of the human catalase gene influences transcription factor binding, reporter gene transcription and is correlated to blood catalase levels. Free Radic Biol Med 2001;30:500–5. 21. Sözmen EZ, Sözmen B, Delen Y, Onai T. Catalase/superoxide dismutase (SOD) and catalase/paraoxanase (PON) ratios may implicate poor glycemic control. Arch Med Res 2001;32:282–7. 22. Sözmen B, Delen Y, Girgin FK, Sözmen EZ. Catalase and paraoxanase in hypertensive type 2 diabetes mellitus. Clin Biochem 1999;32:423–7.

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منابع مشابه

Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for clinical diagnosis.

PCR is increasingly being used as a diagnostic test for the detection of Bordetella pertussis and Bordetella parapertussis DNA, as it has improved sensitivity and specificity in comparison to conventional techniques. The assay described here uses the two insertion sequences IS481 and IS1001 for B. pertussis and B. parapertussis, respectively, with detection by molecular beacons. The real-time P...

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Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis

A triplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis was developed. Three targets were used for amplification in a single tube: the insertion sequence IS481 and the pertussis toxin promoter region (ptxP) for B. pertussis, and the insertion sequence IS1001 for B. parapertussis. The performance of this PCR assay was evaluated in para...

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Novel multitarget real-time PCR assay for rapid detection of Bordetella species in clinical specimens.

A novel multitarget real-time PCR (RT-PCR) assay for the rapid identification of Bordetella pertussis, B. parapertussis, and B. holmesii was developed using multicopy insertion sequences (ISs) in combination with the pertussis toxin subunit S1 (ptxS1) singleplex assay. The RT-PCR targets for the multiplex assay include IS481, commonly found in B. pertussis and B. holmesii; IS1001 of B. parapert...

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Detection and differentiation of Bordetella spp. by real-time PCR.

Molecular detection of Bordetella pertussis DNA is a sensitive and specific method for the rapid diagnosis of pertussis. In this study, a new molecular assay for the detection and differentiation of Bordetella spp. based on automated DNA extraction and real-time PCR was evaluated. The analytical sensitivity of the new assay was determined by Probit analysis of serial dilutions of both cloned PC...

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Multiplex LightCycler PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal specimens.

A rapid real-time multiplex PCR assay for detecting and differentiating Bordetella pertussis and Bordetella parapertussis in nasopharyngeal swabs was developed. This assay (LC-PCR-IS) targets the insertion sequences IS481 and IS1001 of B. pertussis and B. parapertussis, respectively, and is performed using the LightCycler (Roche Molecular Biochemicals, Indianapolis, Ind.). The analytical sensit...

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Development and use of an internal positive control for detection of Bordetella pertussis by PCR.

An internal control of amplification was constructed by recombinant PCR to detect PCR inhibitors. This exogenous DNA was included in the reaction mixture and coamplified with the target gene. This detection was successfully applied to the diagnosis of whooping cough by amplification of a fragment of Bordetella pertussis IS481.

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عنوان ژورنال:
  • Clinical chemistry

دوره 51 12  شماره 

صفحات  -

تاریخ انتشار 2005