Increase the Visibility of Microbial Growth in a Winogradsky Column by Substituting Diatomaceous Earth for Sediment
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16S rRNA Gene Survey of Microbial Communities in Winogradsky Columns
A Winogradsky column is a clear glass or plastic column filled with enriched sediment. Over time, microbial communities in the sediment grow in a stratified ecosystem with an oxic top layer and anoxic sub-surface layers. Winogradsky columns have been used extensively to demonstrate microbial nutrient cycling and metabolic diversity in undergraduate microbiology labs. In this study, we used high...
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Winogradsky columns are model microbial ecosystems prepared by adding pond sediment to a clear cylinder with additional supplements and incubated with light. Environmental gradients develop within the column creating diverse niches that allow enrichment of specific bacteria. The enrichment culture can be used to study soil and sediment microbial community structure and function. In this study w...
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As an investigation that was part of a larger module, a Winogradsky Column was developed to culture a mixed flora of bacteria. A column was created and other additional columns were started with differing conditions to determine the effects of bacterial growth. Extensions were developed to help expand the use of the Winogradsky Column as a teaching tool.
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Laboratory bioassays were carried out to evaluate the insecticidal efficacy of SilicoSec? against 7 - 14 day old adults, old and young larvae of Tribolium castaneum (Herbst) with the mean ? SE (n = 30) weight of 3.4 ? 0.1 and 0.6 ? 0.1 mg, respectively at 27?C and 55 ? 5% RH. Wheat treated with four dose rates of SilicoSec? and untreated wheat served as a control with three replications. Adult?...
متن کاملMicrobial Life in a Winogradsky Column: From Lab Course to Diverse Research Experience†
Appendix 1: List of biochemical and molecular assays utilized in the Winogradsky column lab, as well as purposes and protocol references Appendix 2: 16S-rRNA primers and PCR conditions which grants the public the nonexclusive right to copy, distribute, or display the published work.
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