In vitro Regeneration in Three Varieties of White jute (

نویسنده

  • M. I. Hoque
چکیده

Healthy multiple shoot regeneration was observed from petiole-attached cotyledon (PC) explants of CVL-1 on MS containing 0.2 mg/l BAP and 1.0 mg/l IAA. On the other hand, the best response for multiple shoot regeneration in CVE-3 was obtained when the same explants were cultured on MS with 2.5 mg/l BAP and 0.5 mg/l NAA. However, the same explant of D-154 was found to show less responsive compared to other two varieties to produce multiple shoots. Cotyledonary nodal explants of all the three varieties were found to produce maximum number of multiple shoots on MS supplemented with 0.2 mg/l BAP and 1.0 mg/l IAA. Best root induction was observed at the base of the in vitro regenerated shoots on half the strength of MS supplemented with 0.3 mg/l IBA. The in vitro grown plantlets were successfully transplanted into soil. They grew up to maturity, flowered and fruited like the control plants.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genetic Transformation in White Jute through Agrobacterium and Salinity Screening of Transgenic Plant

The experiment was conducted to establish an efficient and reproducible protocol for the plant regeneration and genetic transformation in white Jute (Corchorus capsularis L.). The regeneration and transformation processes depend on optimum growth conditions, suitable explants and varieties. An attempt was made for Agrobacterium mediated genetic transformation in white jute varieties using gene ...

متن کامل

1835 - 2707 Regeneration and genetic transformation of Tossa Jute ( Corchorus olitorius L . )

The experiment was conducted to establish an efficient and reproducible protocol for the plant regeneration and genetic transformation, which can be used for the improvement of tossa Jute (Corchorus olitorius L.). It was demonstrated that the percent of seeds germination in agar based medium found to be very low (43.1%) , as compared to clinical cotton supported MS liquid medium, which was foun...

متن کامل

DNA fingerprinting of jute germplasm by RAPD.

The genotype characteristic of cultivars was investigated, along with varieties of both of the jute species, Corchorus olitorius and Corchorus capsularis, in the germplasm collection at the Bangladesh Jute Research Institute (BJRI). DNA fingerprinting was generated for 9 different varieties and 12 accessions of jute cultivars by using random amplified polymorphic DNA (RAPD). A total of 29 arbit...

متن کامل

The effect of growth regulators and light on the phenolic content and in vitro regeneration of Aloe vera

Aloe vera L. is one of the most valuable plants in the pharmaceutical, cosmetic, sanitary and food industries. In vitro culture is used for commercial production and due to the abundant application of this plant, extensive research has been performed on the in vitro culture of Aloe vera. For this purpose, the present study was conducted at two stages. At the first stage, the best method of ster...

متن کامل

Determining Genetic Diversity of Some Jute Varieties and Accessions Using RAPD Markers

The genetic diversity of 18 jute genotypes of the two cultivated species Corchorus capsularis L. and C. olitorius L. which include released varieties and collected races, referred to as accessions was evaluated. DNA profiling was generated using sequence independent RAPD markers. A total of 140 scorable loci were observed and a dendrogram was constructed with these markers. The relationship tha...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2007