Regulation of cytochrome P-450 biosynthesis in Saccharomyces cerevisiae: relationship to proposed location of some of adenylate cyclase in mitochondrial membrane.

0 2 4 6 8 Time of incubation at 30°C in shaking water bath (h) Fig. 1. Survival of cytochrome P-450 when shaken aerobically at 30°C in 0.1 M-potassium phosphate bufler: protection by chloramphenicol, cycloheximide, dinitrophenol and semianaerobic conditions Symbols: @ 0.1 M-phosphate buffer + 20% glucose + 1 mMchloramphenicol; A, 0.1 M-phosphate buffer + 20% glucose + 1 m~-2,4-dinitrophenol; 0, 0.1 M-phosphate buffer + 20% glucose; 0, 0.1 M-phosphate buffer; x, 0.1 M-phosphate buffer + 20% glucose + 1 mM-cycloheximide; A, semianaerobic conditions.