Establishment of Blastocyst Production System with Freeze-dried Bull Spermatozoa
نویسنده
چکیده
25 Introduction 25 Materials and methods 27 Results 32 Discussion 35 Chapter 4: Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center 39 Abstract 39 Introduction 39 Materials and methods 40 Results 43 Discussion 47 Chapter 5: Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa 49 Abstract 49 Introduction 49 Materials and methods 51 Results 54 Discussion 59 Chapter 6: Conclusive summary 63
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Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa.
Under optimal freeze-drying conditions, solutions exhibit a cake-like porous structure. However, if the solution temperature is higher than the glass transition temperature of the maximally freeze-concentrated phase (Tg') during drying phase, the glassy matrix undergoes viscous flow, resulting in cake collapse. The purpose of the present study was to investigate the effect of cake collapse on t...
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