Substrate Requirements and Kinetic Analysis of the Cortisol Effects on Uridine Uptake and Incorporation by Mouse Lymphoma P1798 Cells in Vitro1
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چکیده
Cortisol markedly suppressed in vitro utilization of uridine-3 H by corticoid-sensitive mouse lymphoma P1798 cells. Corticoid-resistant cells were not affected. Inhibition did not depend on reduced uptake of exogenous carbohydrate precursors since similar effects were obtained in glucose-free, pyruvate-free medium. Omission of glucose and pyruvate per se did not influence uridine-3H uptake and incorporation by
منابع مشابه
Substrate requirements and kinetic analysis of the cortisol effects on uridine uptake and incorporation by mouse lymphoma P1798 cells in vitro.
Cortisol markedly suppressed in vitro utilization of uridine-3 H by corticoid-sensitive mouse lymphoma P1798 cells. Corticoid-resistant cells were not affected. Inhibition did not depend on reduced uptake of exogenous carbohydrate precursors since similar effects were obtained in glucose-free, pyruvate-free medium. Omission of glucose and pyruvate per se did not influence uridine-3H uptake and ...
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Administration of cortisol to mice bearing the cortisol sensitive or -resistant lines of lymphosarcoma P1 798 resulted in a marked inhibition of incorporation of labeled deoxy nucleoside precursors into tumor DNA only in the sensitive tumor. This effect is one of the earliest biochemical changes see n in lymphosarcoma P1798 following glucocorticoid treatment. It is dependent on both time and do...
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