IntraceUular pH Regulation in Cultured Embryonic Chick Heart Cells Na+-dependent CI-/HCO Exchange
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چکیده
Address reprint requests to Dr. Melvyn Lieberman, Department of Cell Biology, Division of Physiology, Duke University Medical Center, P.O. Box 3709-Y, Durham, NC 27710. Dr. Liu's present address is Department of Medicine, Division of Cardiology, University of Arkansas for Medical Sciences, Mail Slot #532, Little Rock, AR 72205. Dr. Piwnica-Worms' present address is Department of Radiology, Harvard Medical School, Brigham and Women's Hospital, Boston, MA 02115. j. GEN. PHYSIOL. ~ The Rockefeller University Press 9 0022-1295/90/12/1247/23 $2.0
منابع مشابه
Intracellular pH regulation in cultured embryonic chick heart cells. Na(+)-dependent Cl-/HCO3- exchange
The contribution of Cl-/HCO3- exchange to intracellular pH (pHi) regulation in cultured chick heart cells was evaluated using ion-selective microelectrodes to monitor pHi, Na+ (aiNa), and Cl- (aiCl) activity. In (HCO3- + CO2)-buffered solution steady-state pHi was 7.12. Removing (HCO3- + CO2) buffer caused a SITS (0.1 mM)-sensitive alkalinization and countergradient increase in aiCl along with ...
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The purpose of this study was to establish the existence of Na/H exchange in cardiac muscle and to evaluate the contribution of Na/H exchange to pHi regulation. The kinetics of pHi changes in cultured chick heart cells were monitored microfluorometrically with 6-carboxyfluorescein and correlated with Nai content changes analyzed by atomic absorption spectrophotometry; transmembrane H+ movements...
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