Heterologous reconstitution the polyunsaturated fatty acid biosynthetic pathway of Phaeodactylum tricornutum in Arabidopsis thaliana
نویسندگان
چکیده
The genes encoding Δ6 desaturase, Δ6 fatty acid elongase, and Δ5 desaturase from the alga, Phaeodactylum tricornutum, were co-expressed in Arabidopsis thaliana under the control of the Brassica napus napin promoter to produce arachidonic acid (ARA; 20:4 Δ 8, 11, ) and eicosapentaenoic acid (EPA; 20:5 Δ 8, 11, 14, ). ARA and EPA accumulated up to 0.5% and 0.05% of total fatty acids, respectively, in the transgenic A. thaliana. The conversion efficiencies of desaturation steps in n6 (18.6% and 23.8%) and n3 (6% and 9%) were different. The efficiency in n6 pathways was higher than that of in n3 pathways. [Yun-tao Li, Yuan-min Zhu, Chun-hua Fu, Mao-teng Li, Long-jiang Yu. Heterologous reconstitution the polyunsaturated fatty acid biosynthetic pathway of Phaeodactylum tricornutum in Arabidopsis thaliana. Life Sci J 2012;9(4):2561-2565] (ISSN:1097-8135). http://www.lifesciencesite.com. 380
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Isolation and Characterization of the Diatom Phaeodactylum Δ5-Elongase Gene for Transgenic LC-PUFA Production in Pichia pastoris
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