Purification and characterization of infectious myonecrosis virus of penaeid shrimp.

The Penaeid Shrimp Viral Pandemics due to IHHNV, WSSV, TSV and YHV: History in the Americas and Current Status

At least four virus caused pandemics have adversely affected the global penaeid shrimp farming industry since 1980. These viruses in the approximate order of their discovery are Infectious hypodermal and hematopoietic necrosis virus (IHHNV), Yellow Head Virus (YHV), Taura Syndrome Virus (TSV), and White Spot Syndrome Virus (WSSV). The socioeconomic impact of the diseases caused by these viruses...

Infectious myonecrosis virus has a totivirus-like, 120-subunit capsid, but with fiber complexes at the fivefold axes.

Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-A resolution. Results reveal a totivirus-like, 120-...

Historic emergence, impact and current status of shrimp pathogens in Asia.

It is estimated that approximately 60% of disease losses in shrimp aquaculture have been caused by viral pathogens and 20% by bacterial pathogens. By comparison, losses to fungi and parasites have been relatively small. For bacterial pathogens, Vibrio species are the most important while for viral pathogens importance has changed since 2003 when domesticated and genetically selected stocks of t...

Development of real-time PCR assays for detection of white spot syndrome virus, yellow head virus, Taura syndrome virus, and infectious hypodermal and hematopoietic necrosis virus in penaeid shrimp

A non-destructive method based on the polymerase chain reaction for detection of hepatopancreatic parvovirus (HPV) of penaeid shrimp.

Current methods to detect hepatopancreatic parvovirus (HPV) infection of penaeid shrimp depend on invasive techniques that require dissecting the organs infected by this virus. However, sacrificing valuable stocks in order to determine their HPV status can be a drawback in the case of breeding programs. A method was developed for HPV detection by applying a polymerase chain reaction (PCR) assay...