Animal Cytogenetics and Comparative Mapping
نویسندگان
چکیده
ABC Fax + 41 61 306 12 34 E-mail [email protected] www.karger.com © 2004 S. Karger AG, Basel 0301–0171/04/1051–0126$21.00/0 Accessible online at: www.karger.com/cgr Abstract. Nasonia vitripennis is a small parasitic hymenopteran with a 50-year history of genetic work including linkage mapping with mutant and molecular markers. For the first time we are now able to anchor linkage groups to specific chromosomes. Two linkage maps based on a hybrid cross (N. vitripennis × N. longicornis) were constructed using STS, RAPD and microsatellite markers, where 17 of the linked STS markers were developed from single microdissected banded chromosomes. Based on these microdissections we anchored all linkage groups to the five chromosomes of N. vitripennis. We also verified the chromosomal specificity of the microdissection through in situ hybridization and linkage analyses. This information and technique will allow us in the future to locate genes or QTL detected in different mapping populations efficiently and fast on homologous chromosomes or even chromosomal regions. To test this approach we asked whether QTL responsible for the wing size in two different hybrid crosses (N. vitripennis × N. longicornis and N. vitripennis × N.giraulti) map to the same location. One QTL with a major effect was found to map to the centromere region of chromosome 3 in both crosses. This could indicate that indeed the same gene/s is involved in the reduction of wing in N. vitripennis and N. longicornis.
منابع مشابه
Animal Cytogenetics and Comparative Mapping
Supported by grants to T.J.R. from the South African National Research Foundation (NRF; GUN 2053812) and the Wellcome Trust (to T.J.R. and M.A.F.-S.). The work was conducted while V.T. was a postdoctoral fellow at the University of Stellenbosch. His support from the NRF, the University of Stellenbosch and the Russian Fund of Basic Research and Grant 01-2163 from the International Association fo...
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