A cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging.

نویسندگان

  • Qin Wu
  • Qinqin Cheng
  • Siming Yuan
  • Junchao Qian
  • Kai Zhong
  • Yinfeng Qian
  • Yangzhong Liu
چکیده

Multimodal imaging is a highly desirable biomedical application since it can provide complementary information from each imaging modality. We propose a protein engineering-based strategy for the construction of a bimodal probe for fluorescence and magnetic resonance imaging. A recombinant protein was generated by the fusion of a supercharged green fluorescence protein (GFP36+) with a lanthanide-binding tag (dLBT) that can stably bind two Gd3+ ions. The GFP36+-dLBT fusion protein showed strong fluorescence and exhibited efficient contrast enhancement in magnetic resonance imaging. This protein probe improves the MR relaxation more efficiently than Gd-DTPA (gadopentetate dimeglumine). The superior cell-penetrating activity of GFP36+ allows the efficient cellular uptake of this fusion protein and it can thus be used as a cellular imaging probe. Dual imaging was conducted in vitro and in mice. This result indicates that the fusion of different functional domains is a feasible approach for making multi-modal imaging agents.

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عنوان ژورنال:
  • Chemical science

دوره 6 11  شماره 

صفحات  -

تاریخ انتشار 2015